Biopharmaceuticals  Mammalian Cell Culture
 
Media Preparation: Media Filtration
 

A mammalian cell culture medium would typically have very many different components including proteins such as hormones and other growth factors, plant hydrolysates, animal serum etc.

Media with components of animal origin are widely used to grow common cell lines such as CHO and NSO, and can have contaminants such as mycoplasmas and endotoxins which pose a significant problem.

Mycoplasmas can have a ruinous effect on mammalian cells as they can alter every cellular parameter resulting in unreliable data which consequently can compromise the safety of the biological products.

Endotoxins from bacterial cell walls, in significant concentrations, can have a detrimental effect on growth of mammalin cells.

Thus, final sterilization grade filtration to remove bacteria and mycoplasma is very important to media preparation for mammalian cell cultures.

Pre-filtration which can take care of particulate and colloidal contamination is required to enhance the throughput from the final filter.

 
 
Application Area
Application
Filter
Key Product Features
Media Preparation Mycolpasma Retention 0.1 µm gamma Sterilizable 50mm PES Capsule Filter AseptiCap KL/KS- γ 25mm, 50mm  >  Absolute retention
 >  Low protein binding
 >  Very high flow rates
 >  100% Integrity tested
 >  pH Compatibility: 1-10
 >  Total traceability:
      Unique marking on each filter
 >  Individual certificate of quality
0.1 µm gamma Sterilizable PES Capsule Filter AseptiCap KS/KL- γ 1", 2", 5", 8" (with Prefilter)
0.1 µm gamma Sterilizable PES Capsule Filter AseptiCap KS- γ 10", 20", 30" (with Prefilter)
Bacterial Retention 0.2 µm gamma Sterilizable PES Inline Filter AseptiCap KL/KS- γ 25mm, 50mm
0.2 µm gamma Sterilizable PES Membrane Capsule Filter AseptiCap KS/KL- γ 1", 2", 5", 8" (with Prefilter)
0.2 µm gamma Sterilizable PES Membrane Capsule Filter AseptiCap KS- γ 10", 20", 30" (with Prefilter)
0.2 µm gamma Sterilizable PES Membrane Cartridge Filter AseptiSure KS- 5", 10", 20", 30"
Pre-filtration Polypropylene Capsule FilterClariCap PP- 1", 2", 5", 8"  >  Very high retention efficiency     (99.999%)
 >  Heat stable construction
 >  Non media migrating
 >  Wide chemical compatibility
 >  Biologically inert
 >  High Throughputs
 >  pH Compatibility: 1-14
Polypropylene Large Capsule FilterClariCap PP- 10", 20", 30"
Polypropylene Cartridge Filter ClariSure PA- 5", 10", 20", 30"
Microglassfiber Membrane Capsule FilterClariCap GS- 1", 2", 5", 8"
Microglassfiber Membrane Large Capsule Filter
ClariCap GS- 10", 20", 30"
Microglassfiber Cartridge Filters ClariSure GP- 5", 10", 20", 30"



 
 
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  Biopharmaceuticals
- Recombinant Proteins
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 Bioprocessing
  Cell Culture and Fermentation
  - Mammalian Cell Culture
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  - Mammalian Cell Culture Clarification
  - Microbial Fermentation
Broth Clarification
  Downstream Processing
  Aseptic Fill and Finish

 
 
 
 
 
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