Blotting

Life Science Research

Transfer Membranes

mdi offer high quality blotting membranes in convenient formats for highly efficient transfers and excellent results. The range includes PVDF, internally supported nitrocellulose and pure nitrocellulose membranes for protein analysis (Western Blots) and plain and charged nylon membranes for nucleic acid analysis (southern and northern blots). These membranes are available in different pore sizes and dimensions for different application needs and provide high signal intensities and low backgrounds.

Nylon, PVDF and internally supported NC membranes offer high strength for assays where multiple reprobings are required.

Selection Chart

Application	Product Type	Special Features
Western Blotting	Pure Nitrocellulose Membrane Type- SCN	High binding capacities for proteins and nucleic acid molecules Minimum background: High signal to noise ratio Uniform and easy wettability Can be blocked by normal blocking methods Does not bind common protein stains Compatible with colorimetric, radiolabelled, chemiluminescent, fluorescent, and staining detection methods
	Internally supported Nitrocellulose Membrane Type- SCNJ	High binding capacities for proteins and nucleic acid molecules Minimum background: High signal to noise ratio Uniform and easy wettability Can be blocked by normal blocking methods Does not bind common protein stains Compatible with colorimetric, radiolabelled, chemiluminescent, fluorescent, and staining detection methods Internally supported to offer superior handlability
	PVDF Membrane Type- SVF	Very high binding capacities Minimal background: High signal to noise ratio Remains flexible and non-brittle after processing Chemically resistant to harsh reagents making it a convenient matrix for protein sequencing Higher strength than pure nitrocellulose Compatible with all types of detection methods
Southern / Northern Blotting	Positively Charged Nylon-66 Membrane Type- SNNP	Very high binding capacities for nucleic acid molecules Easy wettability Ultra violet cross linkable Chemically resistant; tolerant to alkali fixation
Southern / Northern Blotting	Nylon-66 Membrane Type- SNNPZ

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