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  Genomic DNA Miniprep Kit

The mdi Genomic DNA Miniprep Kit is a fast, economical and easy isolation method of high purity gDNA from animal (mammalian) tissues, blood and bacterial cells. The buffer system provided in the kit allows cell lysis followed by selective binding of gDNA to the spin column.

Purified gDNA is eluted in low-salt buffer or water for variety of downstream applications. This technology also does away with the cumbersome methodologies of phenol extraction. (associated with slurries formation) as well as ethanol precipitation (associated with anion exchange based purification system) for desalting.

 

 



 

Ideal for Genomic DNA Isolation from
 
  • Animal Blood
  • Animal Tissue
  • Rodent Tail
  • Bacteria
 
Applications
 
  • Automated fluorescent sequencing
  • Radioactive sequencing
  • Restriction digestion
  • Cloning
  • PCR
 
Unique Performance Advantages
 
  • High binding capacity
  • High yield with different tissue samples and sample weight (upto 25mg)
  • No precipitation step for high purity gDNA
 
Specifications
 
 
Maximum amount of Tissue
25mg
Maximum Bacterial Cells
2 x 109 cells
Capacity of column reservoir
700µl
Binding capacity of membrane (ds DNA)
50µg
Elution volume
200µl
 
  Performance (gDNA Yield)
 
gDNA Yield Vs Different Tissue Samples  
Type of Tissue
Amount
DNA Yield (µg)
Liver
25 mg
24.4
Kidney
25 mg
30.2
Lung
25 mg
10
Spleen
10 mg
15.4
Mouse Tail
25 mg
15
 
gDNA Yield Vs. Tissue Weight  
 
     
gDNA Yield Vs Different Bacterial Cultures
   
     
Sample
O.D
Cell Count
DNA
Elution (Ist)
DNA
Elution (IInd)
Total DNA
(µg)
DH5α
3.0
App. 2x109
14
8
22
XL-1 Blue
3.9
App. 2x109
14.2
3.6
17.8
Bacillus subtilis
3.0
App. 2x109
15.6
7
22.6
 
   
Ordering Information
               
 
Type
Type
Code
CTGK
CTGK
XX
XX
XX
X
Pack Size
Pack Size
Code
50
0050
250
0250
1000
1000
   
 
CTGK
XX
XX
XX
X
0250


 
 

  
 
 
 
Technical Documents
 
 
       
       
 
 
 
 
 
 
 
 
 
     
     
     
     
   
   
   
   
   

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