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Binding Membranes for Molecular Biology
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mdi binding membranes are uniform, paper thin, white plastic supports, having
specially designed porous structure and binding sites suitable for transfer and
hybridization of biological molecules.
mdi offers a wide range of binding membranes viz. Nitrocellulose, Nylon-66, and
PVDF, exhibiting a range of properties to suit various applications.
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- High binding capacities of the transferred molecules
- Good wettabilty for Nitrocellulose and Nylon-66 membranes. PVDF membranes
are hydrophobic
- Ability to retain the molecules without affecting its biological activity
- Chemical compatibility and mechanical durability under conditions of use
- Ability to be blocked by simple procedures
- High signal to noise ratio
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For special applications, mdi offers internally supported binding membranes which
exhibit very high mechanical strength. |
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Nitrocellulose Membrane Type- SCN and SCNJ |
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| SCN |
| Pure nitrocellulose membrane produced specially for life sciences applications. |
| SCNJ |
| Internally supported to offer superior handlability. |
| Characteristics |
- High binding capacities for proteins and nucleic acid molecules
- Minimum background: High signal to noise ratio
- Uniform and easy wettability
- Can be blocked by normal blocking methods
- Does not bind common protein stains
- Compatible with colorimetric, radiolabelled, chemiluminescent, fluorescent,
and staining detection methods
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| Applications |
- Protein blotting
- Dot and slot blots
- Nucleic acid dot/slot blots
- colony/plaque lifts
- Enzyme immunoassays.
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| Packaging |
Sealed in aluminium bags to maintain hydrophilicity and high protein binding on
prolonged storage. The separators are of pure polyester film to avoid any
contamination of the membrane from the separator. |
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| Protein Binding |
| Figure: |
Protein binding capacities of SCN
membranes with BSA. |
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| mdi Polyvinylidene fluoride (PVDF) membrane is a naturally hydrophobic support
matrix which offers much higher protein binding capacities than that of
Nitrocellulose membrane and also binds difficult to bind proteins such as
glycoproteins. |
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| Characteristics |
- Very high binding capacities
- Minimal background: High signal to noise ratio
- Remains flexible and non-brittle after processing
- Chemically resistant to harsh reagents making it a
convenient matrix for
protein sequencing
- Higher strength than pure nitrocellulose
- Compatible with all types of detection methods
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| Applications |
- Ideal support matrix for protein sequencing
- For high performance, reproducible western blotting
- For protein staining, glycolipid detection and immunoblotting
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| Protein Binding |
| Figure: |
Protein binding capacities of SVF
and SCN membranes with BSA. |
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Nylon-66 Membrane Type - SNNP and SNNPZ |
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| SNNP |
| Internally supported Nylon-66 membranes |
| SNNPZ |
| Positively charged for enhanced binding of negatively charged molecules |
| Characteristics |
- Very high binding capacities for nucleic acid molecules
- Easy wettabilty
- Ultra violet cross linkable
- Chemically resistant; tolerant to alkali fixation
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| Applications |
- Nucleic acid transfers
- Dots/slot blots
- Colony/plaque lifts
- Multiple re probing.
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Type |
Type |
Code |
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SCNJ
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SCNJ |
SVF |
SVFX |
SNNP |
SNNP |
SNNPZ |
SNPZ |
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Size |
Dia |
Code |
82mm |
13 |
90mm |
14 |
137mm |
20 |
142mm |
16 |
80mm x 100mm |
88 |
150mm x 150mm
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87 |
200mm x 200mm |
86 |
300mm x 300mm |
85 |
3M x 300mm |
84 |
3M x 240mm |
83 |
3M x 100mm |
81 |
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Pore Size |
Pore Size |
Code |
0.2µm |
01 |
0.45µm |
02 |
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Sterile/ Non
Sterile |
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Code |
Non Sterile |
1 |
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Pack Size |
Pack Size |
Code |
25 |
11 |
50 |
03 |
Roll |
01 |
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* SVF, SNNP and SNNPZ are available in a maximum width of 240 mm
** SCNJ is available in a maximum width of 100mm |
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