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Membrane for Lateral Flow Tests
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mdi produces highly consistent and optimized Nitrocellulose (NC) membranes for lateral flow tests. A wide variety of membranes are available in roll form as standard products to meet various needs.
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Membrane Type CNPF, CNPC and CNPH-N are directly cast on a transparent polyester backing to improve the handling strength. Standard polyester film is 100µm thick, although other thickness can be available on order.
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Type |
Feature |
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Lower protein binding |
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Higher protein binding |
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Highest protein binding. A high strength, scratch resistant membrane |
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The wicking rate primarily determines the reaction kinetics and is an important characteristic of NC membrane for lateral flow tests. mdi membranes are designated by pore size/wicking rate and have reproducible and defined wicking rates. The wicking rate of blocked membranes will, however depend on the blocking protocol.
Technical data for various membranes.
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Type |
CNPF |
Nominal Pore Size (µm) |
5 |
8 |
10 |
12 |
Membrane thickness (µm) |
100 |
100 |
100 |
100 |
4cm Wicking Time (sec) (blocked) |
185 |
155 |
100 |
95 |
4cm Wicking Time (sec) (unblocked) |
220 |
190 |
130 |
120 |
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Type |
CNPC |
Rating |
10 |
12 |
15 |
Membrane thickness (µm) |
105 |
105 |
105 |
4cm Wicking Time (sec) (blocked) |
120 |
95 |
80 |
4cm Wicking Time (sec) (unblocked) |
140 |
120 |
100 |
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Type |
CNPH-N |
Nominal Pore Size (µm) |
90 |
150 |
200 |
Membrane thickness (µm) |
110 |
110 |
110 |
4cm Wicking Time (sec) (blocked) |
75 |
125 |
165 |
4cm Wicking Time (sec) (unblocked) |
90 |
150 |
200 |
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Selection of membrane depends on the assay. Highest sensitivity is obtained with smaller pore size (slower wicking rate). For high affinity Ag/Ab reaction, use of bigger pore size (faster wicking rates) can result in faster test with adequate sensitivity.
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Pore Size
(Wicking time) |
5,8µm
(180-220 sec) |
10.12µm
(110-150 sec) |
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Characteristics of the test system |
- Highest sensitivity
- Slower migration
- Longer test time
- Sharp line |
- Medium sensitivity
- Medium migration rate
- Medium test time
- Sharp line |
- Lower sensitivity
- Fast migration
- Quick test
- Relatively diffused signal line |
Typical applications |
- Hbs Ag serum
- Urine Ab tests
- Environmental and
Agricultural analytes
- Milk testing
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- hCG
- Heat disease markers
- Cancer markers
- Drugs of Abuse
- Infectious disease tests
- Whole blood tests
- Environmental and agriculture analytes |
- hCG
- Malaria
- Whole blood tests |
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mdi recommends storage of membrane rolls at 15 °C to 20 °C for longer shelf life. The membrane rolls should be kept away from heat radiators. membrane is inflammable when subjected to direct
heat.
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What is Special About mdi Membranes |
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Consistency and stability of NC membranes has long been an issue for manufacturers of membrane based assays. By an optimised surface chemistry and unique processing techniques mdi has resolved these issues. mdi membranes are highly consistent and exhibit extremely low variation. Typical coefficient of variation(CV) for thickness of membrane is 3.5% whereas that for the wicking rate is 4%.
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Typical stability data of mdi membranes |
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As seen in the figure the mdi membranes with detergents are extremely stable.
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| The membrane can be ordered as below: |
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Information |
Membrane Type
with rating
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Width of roll
(in mm)*** |
Length of roll
(in meters) **** |
Quantity |
Example 1 |
CNPF-10μ* |
25mm |
50M or 100M |
20,000 |
Example 2 |
CNPF-10μ* |
20mm |
50M or 100M |
15,000 |
Example 3 |
90CNPH-N* |
25mm |
50M or 100M |
18,000 |
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To order please specify: |
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* The CNPC and CNPF rating is suffixed to membrane type.
** The CNPH-N rating is prefixed to the Membrane type -CNPH-N.
*** The widths are as per the customer requirement. 25mm is most widely used membrane size.
**** The membranes in reels form are supplied on standard 3 inches plastic core. |
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