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  Ni-NTA Hi-Flow Agarose

mdi Ni-NTA agarose combines high binding capacities, stability and reproducibility to provide high performance systems for fast and reliable purification of His-tagged proteins.

Protocol for Use
A detailed protocol for packing, optimization, purification and cleaning is available with each pack.

 



 

Special Features
 
  • High binding capacity for His-tagged proteins
  • Reproducible results in terms of purity selectivity and recovery
  • High stability for fast flow purification
  • Suitable for gravity flow purification as well as instrumentated (HPLC) purification
  • Easy, fast and reliable up-scalability
 
Specifications

 
Gel Matrix
Cross linked 6% spherical agarose beads
Particle Size (Average)
90µm
Dynamic Binding Capacity
Approx. 40mg His-tagged
Metal ion Capacity
15 µmol Ni²+/ml medium
Max. Linear Flow Rate
400-500 cm/hr
Recommended Flow Rate
100-150 cm/hr
Max. Operating Pressure
1 bar
Chemical Stability 0.01 M HCl, 0.1 M NaOH.
Tested for one week at 40°C.
1M NaOH, 70% acetic acid.
Tested for 12 hr.
2% SDS. Tested for 1 hr.
30% 2-propanol. Tested for 30 min.
Storage 20% ethanol at + 4°C to +30°C
Do not freeze
pH Stability 3 - 12 long term (1 week)
2 - 14 short term (1-2 hours)
 
Ordering Information
                 
Product
Type
Pack Size
Ni-NTA Hi-Flow Agarose Gel
NHG10
10ml
NHG25
25ml
NHG50
50ml
NHG100
100ml
NHG250
250ml

To order please specify:

Type
Qty

Example: NHG100, 2 nos

 
 

  
 
 
 
 
 
       
       
 
 
 
 
 
 
 
 
 
     
     
     
     
   
   
   
   
   

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