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  PCR Purification Kit

mdi PCR Purification Kit is a fast and economical way to purify DNA fragments (100bp-10Kb) from PCR amplification reactions in small elution volume.

The mdi PCR purification system combines specially developed mdi membrane, reagents and the convenient spin column technology to capture target DNA. The contaminants such as enzymes, primers, nucleotides etc. are washed away and the bound target DNA is eluted in a low salt concentration elution buffer.



 



 

Applications
 
  • Ligation
  • Transformation/ Transduction/ Transfection
  • Automated fluorescent sequencing
  • Radioactive sequencing
  • Restriction Digestion
  • Labelling
  • Cloning
 
Specifications
 
 
Range of DNA fragments that can be isolated
100bp-10Kb
10µg
Recovery
90-95%
(100bp-10Kb)
Recommended elution volume
30µl
Total time taken
6 minutes
 
  Performance (pDNA Yield)
 
Complete primer removal using
mdi PCR Purification Kit
a: Before purification b: After purification
M: Marker Samples were analyzed on a 0.8% TAE agarose gel.
 
 
Ordering Information
               
 
Type
Type
Code
SPCK
SPCK
XX
XX
XX
X
Pack Size
Pack Size
Code
50
0050
250
0250
1000
1000
   
 
SPCK
XX
XX
XX
X
0250


 
 

  
 
 
 
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