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	Whole Blood Separator 'point of care testing'

Advancements in blood separation research at mdi as resulted in a unique set of red blood cell (RBC) separation materials which satisfy the long standing demands of the lateral flow diagnostic test manufacturing industry.

Special Features

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Blood separators are useful as they offer following advantages to a point of care rapid test manufacturer.
Eliminate the need for storage, centrifugation or clotting of blood samples No technical expertise and lab equipment is required True one step mathod. No liquid buffer is required in the kit On the spot results in critical situations Results are reliable as intermixing of patient's sample is avoid

More Information

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Commonly Faced Problems with Blood Separators	Solutions offer by mdi Blood Separator WFR1
Leakage of RBC on the membrane (specially at high hematocrit level)	Higher void volume and extremely high retention efficiency results in full stoppage of RBC
The migration of plasma on the membrane is very slow resulting in delayed results	The unique composite design facilitates faster migration of plasma through the separator
High volume of blood is required due to poor plasma recovery	The unique composite structure allows high plasma recovery making it possible to make one step whole blood assays with as little as 25µl whole blood
The separator may work for fingerprick blood but does not work with stored blood (with anticoagulant)	mdi separator work well with fingerprick as well as stored blood
The separator adsorbs the analyte resulting in reduced sensitivity	The adsorption of the analyte is minimal resulting in higher sensitivity

Modes of Whole Blood Test Kits

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Whole blood lateral flow tests are designed in two distinct modes.

Mode 1

Mode 2

A liquid buffer is provided with the test kit to drive the plasma through the sample pad and the membrane to complete the assay. Minimal quantity of whole blood can be used.

The test kit does not contain any liquid buffer. Plasma separated from applied whole blood itself
Migrates up the membrane to complete the assay.

Format Design

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Mode 1

Blood separator types FR1 and FR2 act as the sample pad and will stop the RBC. Typically 5-50µl whole blood is applied in the hole of the device and 2-3 drops of buffer are added to drive the plasma. A separate conjugate release pad is required.

Figure 1.0

Blood Volume Required in Mode 1

Type	With Buffer		Without Buffer
Type	Min	Max	Min	Max
FR1	20µl	45µl	45µl	70µl
FR2	-	-	65µl	120µl

Mode 2

The polyester matrix of WFR1stops some RBC allowing free flow of plasma whereas the white matrix stops the remaining RBC completely allowing only plasma to flow through the NC membrane. Due to the design and construction of above material the RBC do not leak to the membrane assuring a valid assay, even if excess blood is applied. To conserve plasma and enhance the speed of flow, WFR1 also acts as conjugate release matrix. The format design is shown in Figure 2. Typically 2-3 drops of whole blood is required. However, by proper design of a dipstick with reduced dimensions, tests can be made to run with only one drop of whole blood.

Cellulose Nitrate Membrane Disc Filter

Figure 2.0

Comparative Performance of WFR1 with Competing Blood Separator

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	Time for plasma to reach window of device (sec)	Time for plasma to cross window (sec)	Hemolysis	Volume of blood sample added
WFR1 - mdi	85	152	No	3 drops
Competition - C	250	360	Seen after 10 min in some samples	4drops

Blood Separator Type FR2

With lower RBC separation capacity than FR1, for applications with smaller blood sample, FR2 is the cost effective solution.

Ordering Information

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FR1 and FR2 are available in roll form slit to desired width with 0.35mm and 0.60 mm thickness.
WFR1 is available only in strip form up to 30 cm long. Width is customer specified as require to fit the cassette/dipsticks.