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  pDNA Miniprep Kit (Less than 30 minutes)
 

mdi Miniprep Kit is a fast and economical way to obtain high yield (up to 20µg) of high purity pDNA from bacterial cultures.

This kit provides high yields of pure DNA, predominantly in super coiled form, in small elution volumes.

The mdi Miniprep system makes use of the ability of the unique mdi membrane to capture pDNA along with the convenient spin column technology. The bound plasmid DNA is eluted in small volumes of very low salt concentration buffer.

DNA Purity
pDNA purified with mdi Express Miniprep exhibits an A260/ A280 ratio of 1.8-2.0 in 10mM Tris-Cl, pH 8.5.

 
Applications
 
 
Specifications
 
Type
Feature
Capacity of column reservoir
700µl
20µg
Yield
80%
Elution volume
50µl
Total time taken
30 minutes
Culture volume
1-5ml
 
  Performance (pDNA Yield)
 
mdi pDNA yield which can be quantified in terms of concentration as well as total recovery varies depending on plasmid copy number per cell, the individual insert in a plasmid , factors that affect growth of the bacterial culture, the elution volume and the elution incubation time. mdi miniprep kit has been characterized for optimal yields with respect to elution volumes as well as culture volumes.



Comparison

mdi miniprep characterization as well as yield comparisons were carried out with a high copy number plasmid of 11.5 Kbp length.

mdi Vs. Competitor Q
Comparison with identical protocols to competition Q
Ordering Information
               
 
Type
Type
Code
MIPK
MIPK
XX
XX
XX
X
Pack Size
Pack Size
Code
50
0050
250
0250
1000
1000
   
 
MIPK
XX
XX
XX
X
0250


 
 

  
 
 
 
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