Endotoxin Free Quanta Midi Kit
 

mdi Endotoxin Free Quanta Midi Kit is uniquely designed to facilitate ultrapure pDNA free from endotoxins (<0.1EU/µg), ideal for transfection even into highly sensitive mammalian cells. These kits offer high yields from both low copy as well as high copy number plasmid. The innovative buffer system and filter device provides efficient binding of pDNA onto the spin column.

  Quanta Midi Column
 
 
 
  • Special Features
  • Applications
  • Specifications
  • Protocol
  • Ordering Information
  • Extremely low Endotoxin levels, <0.1 EU/μg
  • Fastest pDNA isolation in <30 minutes
  • Very high binding capacities and yields in miniaturized spin columns
  • Highly concentrated ultrapure pDNA: more than 1μg/μl
  • Eluted pDNA is ideal for transfection into highly sensitive mammalian cell lines
  • No salt contamination due to isopropanol precipitation
  • No yield losses due to DNA reconstitution
  • No gravitational waiting
  • Simple filtration to remove cell debris; No centrifugation
  • High within lot as well as lot to lot consistency
  • Automated Fluorescent Sequencing
  • Radioactive Sequencing
  • Restriction Digestion
  • Transfection (with highly sensitive mammalian cell lines)
  • Cloning
  • PCR
Specifications
   High Copy Plasmid Low Copy Plasmid
  Binding capacity of membrane ( ds DNA) 400 μg 400 μg
  Recovery 80% 80%
  Maximum culture volumes 25-35 ml 50 ml
  Expected yield of plasmid 150-250 μg 100-200 μg
Assurance
  Ultrapure Plasmid DNA   A260 / A280 = 1.8-2.0
  Quality Management System   ISO-9001:2008 Certified
 
 
Endotoxin Free Quanta Midi Kit Protocol
Type
QDEK
QDEK
 
XX
 
XX
 
XX
 
X
 
Pack Size
Pack Size
Code
25
0025
100
0100
 
 
QDEK
XX
XX
XX
X
0100

 
 
 
 
 
 
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  Related Documents
  -  User Guide
  -  Certificate of Quality

  Related Products
  -  FastLyse pDNA Miniprep Kit
  -  pDNA Miniprep Kit
  -  Express 96 pDNA Miniprep Kit
  -  Quanta Maxi Kit
  -  Quanta Midi Kit
  -  Endotoxin Free Quanta Maxi Kit
 
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